Introduction. To examine the amounts and role of growth factors in different tissues and corporal fluid, new sensitive techniques have to be developed. A major problem is that the normal concentration of trophic substances, such as nerve growth factor (NGF), in central and peripheral nervous system and in fluids is very low (ng-pg/ml). A valuable method of research is the sensitive two-site enzyme immunoassay using the monoclonal antibody 27/21 to mouse NGF. Materials and methods. The present work applied this enzyme immunoassay to examine the NGF levels in normal non-human primate sera (n= 94) and applied this assay to study of NGF levels in two non-human primate receiving NGF infusion: one young and one aged. Two groups of non-human primate sera were studied one young-adult (n= 69) and one aged (n= 25). The serum samples NGF treated non-human primate were taken before the infusion and at the 1st week and 1st, 3rd, 6th and 12th month after infusion. Results. To further test the specificity of conjugate binding, dilutions of the non-human primate sera were preincubated with an excess of monoclonal NGF antibody 27/21 in solution. With this strategy it was possible to completely block the signal obtained using the enzyme immunoassay. We found very low levels of NGF in aged monkeys (0.054 ng/ml) when compared with young-adult group (0.152 ng/ml) (p> 0.01). The NGF levels in aged non-human primate treatment with NGF was very low before (0.50 ng/ml) and during NGF treatment evolution time, whereas at the the 12th month showed an increase in NGF levels (0.180 ng/ml). We found normal values of NGF in the young monkey before and during the first year after NGF infusion. Conclusions. Using the enzyme immunoassay described it is possible to know the serum concentration of NGF immunoreactive in non-human primate and this assay is able to detect peripheral changes in NGF levels after intracerebral infusion of NGF.
KeywordsAlzheimer’s diseaseEnzyme immunoassayIntracerebral infusionNerve growth factorNon-human primateCategoriesDemenciaNeurodegeneraciónNeurogeriatríaNeuropsiquiatríaPatología vascular
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