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The levels of expression of toll-like receptors 2 and 4 in neutrophils are associated with the prognosis of ischaemic stroke patients

D. Brea, M. Blanco, T. Sobrino, P. Ramos-Cabrer, J. Castillo [REV NEUROL 2011;52:12-19] PMID: 21246489 DOI: https://doi.org/10.33588/rn.5201.2010494

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Volumen 52 | Number 01 | Nº of views of the article 5.674 | Nº of PDF downloads 496 | Article publication date 01/01/2011

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ABSTRACT

INTRODUCTION Cerebral ischaemia triggers a very intense inflammatory response that is characterised by the infiltration of leukocytes in the brain parenchyma. The first of these cells to reach the brain are the neutrophils. Nevertheless, the role played by neutrophils and the expression of their innate immunity receptors TLR2 and TLR 4 (toll-like receptors 2 and 4) in cerebral ischaemia is still not fully understood.

AIM To examine the association between the levels of neutrophils and the expression of TLR2 and TLR4 in those cells and the prognosis of ischaemic stroke patients.

PATIENTS AND METHODS The study involved a sample of 110 patients with an ischaemic stroke that had started less than 12 hours earlier. The main variables that were taken into account were the modified Rankin scale at three months (poor prognosis > 2) and the volume of the infarction (measured by means of computerised tomography between days 4 and 7). We analysed the percentage of neutrophils and the mean expression of TLR2 and TLR4 in neutrophils by means of flow cytometry on admission, at 24 h, at 72 h and at 7 days.

RESULTS The TLR4 expression in neutrophils at 72 h (odds ratio, OR = 2.1; confidence interval of 95%, CI 95% = 1.4-3.2) and at 7 days (OR = 3.2; CI 95% = 1.7-6.1) was associated independently with the patients’ prognosis and with the volume of the infarction at 72 h (B = 5.4; CI 95% = 2.9-7.8) and at 7 days (B = 7.0; CI 95% = 4.5-9.6).

CONCLUSIONS Neutrophils are associated with the prognosis of patients with ischaemic stroke through the expression of their TLR4. KeywordsCerebral ischaemia Inflammation Neutrophils Prognosis Stroke Toll-like receptors CategoriesPatología vascular

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Original

The levels of expression of toll-like receptors 2 and 4 in neutrophils are associated with the prognosis of ischaemic stroke patients