NRTI
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M41L, L74V, M184V, L210W, T215Y
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3TC, ABC, AZT, D4T, DDI, FTC, TDF
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–
|
M41L, L74V, M184V, L210W, T215Y
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3TC, ABC, AZT, D4T, DDI, FTC, TDF
|
–
|
PI
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M46L, I54V, V82A, I84V
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ATV/r, FPV/r, IDV/r, LPV/r, NFV, SQV/r, TPV/r
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DRV/r
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M46L, I54V, I84V
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ATV/r, FPV/r, IDV/r, LPV/r, NFV, SQV/r, TPV/r
|
–
|
a Plasma resistance tests were performed in 2008, before tenofovir/emtricitabine + darunavir/ritonavir + raltegravir regime was instituted. Cerebrospinal fluid resistance tests were performed in 2013, before the addition of etravirine and maraviroc to the previous regime. All virology assays were performed in the Laboratory of Molecular Virology of our center as follows: Viral load with RealTime HIV-1 Abbott Molecular Inc. ARV genotypic resistance test with ViroSeq TM HIV-1 Genotyping System v2.0 Celera corporation, with a modification in entry volume to 1.0 mL, and centrifugation parameters (initial time 2 h, 17000 rpm, 4 °C), volume adjusted to 25 µL for RNA suspension when viral load < 1000 copies/mL. Interpretation of the resistance pattern was performed with the Stanford HIV db Program Genotypic Resistance Interpretation Algorithm v7.0. HIV-1 co-receptor tropism assays were performed by using HIV-1 V3 loop amplicons with primers as described by the HIV French Resistance Group [9], with the interpretation algorithm Geno2Pheno v2.5. 3TC: lamivudine; ABC: abacavir; AZT: zidovudine; D4T: stavudine; DDI: didanosine; FTC: emtricitabine; TDF: tenofovir; EFV: efavirenz; NVP: nevirapine; ETR: etravirine; RPV: rilpivirine; ATV/r: atazanavir/ritonavir; FPV/r: fosamprenavir/ritonavir; IDV/r: indinavir/ritonavir; LPV/r: lopinavir/ritonavir; NFV: neflvinavir; SQV/r: saquinavir/ritonavir; TPV/r: tipranavir/ritonavir; DRV/r: darunavir/ritonavir.
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